Journal: International Journal of Legal Medicine

Article Title: Characterization of degradation and heterozygote balance by simulation of the forensic DNA analysis process

doi: 10.1007/s00414-016-1453-x

Figure Lengend Snippet: Concentrations (ng/ μ l), degradation index ( DI ), and estimated degradation parameter ( P ( d e g )) for nine degraded tissue samples based on quantification by Quantifiler®; Trio DNA Quantification Kit (QT) and PowerQuant TM System (PQ). Target amplicon size in base pairs is 80 and 84 for the small targets, and 214 and 294 for the large targets. A degradation index DI is calculated by dividing the small autosomal target DNA concentration by the large autosomal target DNA concentration (Eq. 2 )

Article Snippet: The Quantifiler®; Trio DNA Quantification Kit (Applied Biosystems), with an 80 bp small autosomal target and 214 bp large autosomal target, and PowerQuant TM System (Promega), with an 84 bp small autosomal target and 294 bp large autosomal target, were used for quantification.

Techniques: Amplification, Concentration Assay